ARG52756
anti-CD34 antibody [QBEND/10]
anti-CD34 antibody [QBEND/10] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Primates,Rabbit
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker) |
|---|---|
| Tested Reactivity | Hu, Ms, NHuPrm, Rb |
| Species Does Not React With | Rat, Bov, Dog, Sheep |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | QBEND/10 |
| Isotype | IgG1, kappa |
| Target Name | CD34 |
| Antigen Species | Human |
| Immunogen | Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta. |
| Conjugation | Un-conjugated |
| Alternate Names | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Lung, Tonsil | ||||||||||||||
| Observed Size | 105-120 kDa |
Properties
| Form | Liquid |
|---|---|
| Buffer | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and <0.1% Sodium azide |
| Preservative | <0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34 |
|---|---|
| Gene Symbol | CD34 |
| Gene Full Name | CD34 molecule |
| Background | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| Cellular Localization | Cytoplasm, Membrane |
| Highlight | Related Antibody Duos and Panels: ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34) ARG30328 Angiogenesis Antibody Panel Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Vascular development is regulated by FGF-dependent metabolic control |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| Calculated MW | 41 kDa |
| PTM | Highly glycosylated. Phosphorylated on serine residues by PKC. |
Images (7) Click the Picture to Zoom In
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ARG52756 anti-CD34 antibody [QBEND/10] ICC/IF image
Immunofluorescence: hCMEC/D3 cells stained with ARG52756 anti-CD34 antibody [QBEND/10].
From Lucie Y Li et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1077204, Fig. 3A & B.
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ARG52756 anti-CD34 antibody [QBEND/10] WB image
Western blot: 30 µg of mouse lung lysate stained with ARG52756 anti-CD34 antibody [QBEND/10] at 1:500 dilution.
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Breast Ductal Carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Cervical Squamous Cell carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Colon stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Placenta stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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Review for anti-CD34 antibody [QBEND/10]
Application：WB
Sample：Mouse lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Brain blood vessel autoantibodies in patients with NMDA and GABAA receptor encephalitis: identification of unconventional Myosin-X as target antigen
ICC/IF / Human
Role of Angiogenesis in Chronic Radiation Proctitis: New Evidence Favoring Inhibition of Angiogenesis Ex Vivo.
IHC-P / Human
