ARG65401
anti-CD328 / Siglec 7 antibody [6-434]
anti-CD328 / Siglec 7 antibody [6-434] for CyTOF®-candidate,Flow cytometry and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [6-434] recognizes CD328 / Siglec 7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS |
| Specificity | The mouse monoclonal antibody 6434 recognizes CD328 (Siglec7), a 75 kDa transmembrane glycoprotein expressed mainly on NK cells, dendritic cells and monocytes. HLDA 8; WS Code ARN80652 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6-434 |
| Isotype | IgG1 |
| Target Name | CD328 / Siglec 7 |
| Antigen Species | Human |
| Immunogen | human dendritic cells |
| Conjugation | Un-conjugated |
| Alternate Names | QA79 membrane protein; p75; CD antigen CD328; SIGLEC19P; Adhesion inhibitory receptor molecule 1; SIGLECP2; Sialic acid-binding Ig-like lectin 7; SIGLEC-7; p75/AIRM1; CDw328; Siglec-7; AIRM-1; CD328; AIRM1; D-siglec; QA79 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y286 Human Sialic acid-binding Ig-like lectin 7 |
|---|---|
| Gene Symbol | SIGLEC7 |
| Gene Full Name | sialic acid binding Ig-like lectin 7 |
| Background | CD328, also known as Siglec-7 or p75/AIRM1, is a 75 kDa type I transmembrane glycoprotein of sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD328 binds to sialylated glycans with alpha2,6 sialyl and alpha2,8 disyalyl residues and mediates sialic acid-dependent cell-cell binding. As it contains in its intracellular part the immunoreceptor tyrosine-based inhibitory motif (ITIM), it serves as an inhibitory receptor, e.g. of NK cells. |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). [UniProt] |
| Highlight | Related products: CD328 antibodies; CD328 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody |
| Calculated MW | 51 kDa |
| PTM | Tyrosine phosphorylated. |
