ARG20905

anti-CD31 antibody [390] (APC)

Datasheet

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50 μg

315.00

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Datasheet

Overview

Product Description	APC-conjugated Rat Monoclonal antibody [390] recognizes CD31
Tested Reactivity	Ms
Tested Application	BL, FACS, ICC/IF, IHC-Fr
Specificity	Mouse CD31. The clone 390 inhibits the aggregation of L cells transfected with a variant form of CD31.
Host	Rat
Clonality	Monoclonal
Clone	390
Isotype	IgG2a, kappa
Target Name	CD31
Antigen Species	Mouse
Immunogen	C3H/HeJ mouse hematopoietic progenitor cell line 32D
Conjugation	APC
Alternate Names	EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule

Application Instructions

Application Suggestion

Tested Application	Dilution
BL	Assay-dependent
FACS	< 0.2 µg/10^6 cells
ICC/IF	Assay-dependent
IHC-Fr	Assay-dependent

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	PBS, 0.1% Sodium azide and Sucrose.
Preservative	0.1% Sodium azide
Stabilizer	Sucrose
Concentration	0.1 mg/ml
Storage Instruction	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 18613 Mouse PECAM1 Swiss-port # Q08481 Mouse Platelet endothelial cell adhesion molecule
Gene Symbol	PECAM1
Gene Full Name	platelet/endothelial cell adhesion molecule 1
Background	CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Function	CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt]
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
Calculated MW	83 kDa
PTM	Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.