ARG56068
anti-CD31 antibody [1A10]
anti-CD31 antibody [1A10] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [1A10] recognizes CD31 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A10 |
| Isotype | IgG1, kappa |
| Target Name | CD31 |
| Antigen Species | Human |
| Immunogen | Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of CD31. |
| Conjugation | Un-conjugated |
| Alternate Names | EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | THP-1 | ||||||
| Observed Size | ~ 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified high titer supernatant |
| Buffer | IMDM, 0.05% Sodium azide and 5% FBS |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% FBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P16284 Human Platelet endothelial cell adhesion molecule |
|---|---|
| Gene Symbol | PECAM1 |
| Gene Full Name | platelet/endothelial cell adhesion molecule 1 |
| Background | CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| Function | CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt] |
| Cellular Localization | Cell surface and cytoplasm of endothelial cells |
| Highlight | Related products: CD31 antibodies; CD31 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Besides tumor suppression, what’s p53 busy for during embryogenesis? |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody |
| Calculated MW | 83 kDa |
| PTM | Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
Images (2) Click the Picture to Zoom In
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ARG56068 anti-CD31 antibody [1A10] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56068 anti-CD31 antibody [1A10].
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ARG56068 anti-CD31 antibody [1A10] WB image
Western blot: THP-1 cell lysate stained with ARG56068 anti-CD31 antibody [1A10].
Specific References
Immunohistochemical analysis on aquaporin-1 and aquaporin-3 in skin wounds from the aspects of wound age determination.
IHC-P / Human
