ARG42326
anti-CD305 / LAIR1 antibody [NKTA255] (APC)
anti-CD305 / LAIR1 antibody [NKTA255] (APC) for Flow cytometry and Human
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [NKTA255] recognizes CD305 / LAIR1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NKTA255 |
| Isotype | IgG1 |
| Target Name | CD305 / LAIR1 |
| Antigen Species | Human |
| Immunogen | Activated NK cells and CD3- thymocytes. |
| Conjugation | APC |
| Alternate Names | LAIR-1; hLAIR1; CD305; CD antigen CD305; Leukocyte-associated immunoglobulin-like receptor 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6GTX8 Human Leukocyte-associated immunoglobulin-like receptor 1 |
|---|---|
| Gene Symbol | LAIR1 |
| Gene Full Name | leukocyte-associated immunoglobulin-like receptor 1 |
| Background | The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK. N-glycosylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) at 10 µl / 100 µl of peripheral whole blood.
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ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) FACS image
Flow Cytometry: Human lymphocytes stained with ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) at 10 µl / 100 µl of peripheral whole blood and ARG53783 anti-CD19 antibody [LT19] (PE) at 20 µl / 100 µl of peripheral whole blood.
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ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) FACS image
Flow Cytometry: Separation of Human CD305 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG42326 anti-CD305 / LAIR1 antibody [NKTA255] (APC) at 10 µl / 100 µl of peripheral whole blood.
