ARG65354
anti-CD28 antibody [CD28.2] (low endotoxin)
anti-CD28 antibody [CD28.2] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Primates
Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [CD28.2] recognizes CD28 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| Specificity | The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. HLDA V.; WS Code 5T CD28.05 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CD28.2 |
| Isotype | IgG1 |
| Target Name | CD28 |
| Antigen Species | Human |
| Immunogen | DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA |
| Conjugation | Un-conjugated |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: T cell costimulation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28 |
|---|---|
| Gene Symbol | CD28 |
| Gene Full Name | CD28 molecule |
| Background | CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 25 kDa |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. |
Images (2) Click the Picture to Zoom In
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ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) at 1.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) FACS image
Flow Cytometry: Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) at 1.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.
FuncSt / Human
NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis.
Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.
Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling.
