ARG55930
anti-CD274 / PD-L1 antibody
anti-CD274 / PD-L1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD274 / PD-L1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody has no cross-reactivity to PD-L2. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD274 / PD-L1 |
| Immunogen | Synthetic peptide (17 aa) within aa. 60-110 of Human CD274 / PD-L1. |
| Conjugation | Un-conjugated |
| Alternate Names | Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Raji cell lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD274 |
| Gene Full Name | CD274 molecule |
| Background | CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt] |
| Cellular Localization | Cell membrane and Endomembrane system. |
| Highlight | Related products: PD-L1 antibodies; PD-L1 ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: Why anti-PD-1/PD-L1 therapy doesn’t work? The best solution for PD-1/PD-L1 research Examining CTL/NK-mediated cytotoxicity by ELISA |
| Calculated MW | 33 kDa |
Images (5) Click the Picture to Zoom In
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ARG55930 anti-CD274 / PD-L1 antibody WB image
Western blot: 20 µg of Raw264.7 cells untreated or treated with LPS. The blots were stained with ARG55930 anti-CD274 / PD-L1 antibody at 1:1000 dilution.
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ARG55930 anti-CD274 / PD-L1 antibody FACS image
Flow Cytometry: A-20 cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 0.5 µg/ml dilution (red); Isotype control (green).
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ARG55930 anti-CD274 / PD-L1 antibody IHC-P image
Immunohistochemistry: Human tonsil tissue stained with ARG55930 anti-CD274 / PD-L1 antibody at 5 µg/ml.
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ARG55930 anti-CD274 / PD-L1 antibody WB image
Western blot: 10 µg of lysate from HeLa cell transfected with 1) siPD-L1 or 2) control siRNA stained with ARG55930 anti-CD274 / PD-L1 antibody at 1:500 dilution.
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ARG55930 anti-CD274 / PD-L1 antibody IF image
Immunofluorescence: Human Heart cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 20 µg/ml dilution.
Customer's Feedback
Good
Review for anti-CD274 / PD-L1 antibody
Application：WB
Sample：HeLa and Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-CD274 / PD-L1 antibody
Application：WB
Sample：Raw264.7 cells untreated or treated with LPS
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-CD274 / PD-L1 antibody
Application：WB
Sample：HepG2 and HCT116
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Triptolide suppresses oral cancer cell PD-L1 expression in the interferon-γ-modulated microenvironment in vitro, in vivo, and in clinical patients.
WB / Human
Analysis of Blimp-1 and PD-1/PD-L1 Immune Checkpoint in an Autoimmune Thyroiditis Animal Model.
WB, IHC-P / Mouse
