ARG42322
anti-CD273 / PD-L2 antibody [24F.10C12] (APC)
anti-CD273 / PD-L2 antibody [24F.10C12] (APC) for Flow cytometry and Human
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [24F.10C12] recognizes CD273 / PD-L2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 24F.10C12 |
| Isotype | IgG2a, kappa |
| Target Name | CD273 / PD-L2 |
| Antigen Species | Human |
| Immunogen | Human CD273. |
| Conjugation | APC |
| Alternate Names | B7DC; bA574F11.2; PDL2; CD antigen CD273; PD-L2; Programmed cell death 1 ligand 2; CD273; Btdc; PDCD1L2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDCD1LG2 |
| Gene Full Name | programmed cell death 1 ligand 2 |
| Function | Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). [UniProt] |
| Cellular Localization | Isoform 3: Secreted. Isoform 2: Endomembrane system; Single-pass type I membrane protein. Isoform 1: Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 31 kDa |
Images (2) Click the Picture to Zoom In
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ARG42322 anti-CD273 / PD-L2 antibody [24F.10C12] (APC) FACS image
Flow Cytometry: Human stimulated monocytes (GM-CSF + IL-4) stained with ARG42322 anti-CD273 / PD-L2 antibody [24F.10C12] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension.
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ARG42322 anti-CD273 / PD-L2 antibody [24F.10C12] (APC) FACS image
Flow Cytometry: Separation of Human dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Cells were stained with ARG42322 anti-CD273 / PD-L2 antibody [24F.10C12] (APC) at 10 µl / 100 µl of peripheral whole blood.
