ARG66197
anti-CD20 antibody [SQab1719]
anti-CD20 antibody [SQab1719] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1719] recognizes CD20 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, IP |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1719 |
| Isotype | IgG |
| Target Name | CD20 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of CD20. |
| Conjugation | Un-conjugated |
| Alternate Names | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MS4A1 |
| Gene Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Background | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| Function | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30325 Inflammatory Cell Antibody Panel ARG30334 Tumor-infiltrating Lymphocyte Antibody Panel Related products: CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated. Might be functionally regulated by protein kinase(s). |
Images (5) Click the Picture to Zoom In
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ARG66197 anti-CD20 antibody [SQab1719] ICC/IF image
Immunofluorescence: Raji cells fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66197 anti-CD20 antibody [SQab1719] (green) at 1:1000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
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ARG66197 anti-CD20 antibody [SQab1719] FACS image
Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66197 anti-CD20 antibody [SQab1719] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66197 anti-CD20 antibody [SQab1719] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human appendix tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
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ARG66197 anti-CD20 antibody [SQab1719] IP image
Immunoprecipitation: 0.4 mg of Raji whole cell lysate was immunoprecipitated (1:20 dilution) and stained with ARG66197 anti-CD20 antibody [SQab1719].
Lane 1: Immunoprecipitation in Raji whole cell lysate
Lane 2: PBS instead of Primary Ab in Raji whole cell lysate
Lane 3: Raji whole cell lysate, 10 µg (input) -
ARG66197 anti-CD20 antibody [SQab1719] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human spleen tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
