ARG66759
anti-CD2 antibody [SQab20192]
anti-CD2 antibody [SQab20192] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20192] recognizes CD2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20192 |
| Isotype | IgG |
| Target Name | CD2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human CD2. |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Jurkat IHC-P: T-cell lymphoma tissue |
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| Observed Size | 43 ~ 47 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD2 |
| Gene Full Name | CD2 molecule |
| Background | The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene. [provided by RefSeq, Jun 2016] |
| Function | CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 39 kDa |
Images (2) Click the Picture to Zoom In
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ARG66759 anti-CD2 antibody [SQab20192] WB image (Customer's Feedback)
Western blot: 10 µg of Jurkat cell lysate stained with ARG66759 anti-CD2 antibody [SQab20192] at 1:1000 dilution.
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ARG66759 anti-CD2 antibody [SQab20192] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human T-cell lymphoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66759 anti-CD2 antibody [SQab20192] at 18°C - 25°C for 30 minutes.
Customer's Feedback
Excellent
Review for anti-CD2 antibody [SQab20192]
Application：WB
Sample：Jurkat
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
