ARG56009
anti-CD195 / CCR5 antibody [12D1]
anti-CD195 / CCR5 antibody [12D1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [12D1] recognizes CD195 / CCR5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12D1 |
| Isotype | IgG2a, kappa |
| Target Name | CD195 / CCR5 |
| Antigen Species | Human |
| Immunogen | Human native CD195 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCR5 |
| Gene Full Name | chemokine (C-C motif) receptor 5 (gene/pseudogene) |
| Background | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015] |
| Function | Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt] |
| Cellular Localization | Cell surface |
| Calculated MW | 41 kDa |
| PTM | Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. |
Images (5) Click the Picture to Zoom In
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ARG56009 anti-CD195 / CCR5 antibody [12D1] ICC/IF image
Immunofluorescence: PFA-fixed HeLa cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (green).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human stomach tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG56009 anti-CD195 / CCR5 antibody [12D1].
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: PFA-fixed HeLa cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: MCF7 cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: U-87 MG cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
