ARG65523
anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin)
anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [NOK-1] recognizes CD178 / Fas Ligand |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IP, WB |
| Specificity | The mouse monoclonal antibody NOK1 recognizes CD178 / FasL, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells. HCDM Workshop: VII 70322 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NOK-1 |
| Isotype | IgG1 |
| Target Name | CD178 / Fas Ligand |
| Antigen Species | Human |
| Immunogen | L5178Y mouse T lymphoma cells expressing recombinant human CD178 |
| Conjugation | Un-conjugated |
| Alternate Names | FASLG; Fas Ligand; APT1LG1; TNFSF6; CD178; FasL; Tumor Necrosis Factor Ligand Superfamily Member 6; Fas Ligand (TNF Superfamily, Member 6); Apoptosis Antigen Ligand; Fas Antigen Ligand; CD95 Ligand; CD95-L; CD95L; APTL; FASL; Tumor Necrosis Factor (Ligand) Superfamily, Member 6; Mutant Tumor Necrosis Factor Family Member 6; Apoptosis (APO-1) Antigen Ligand 1; Tumor Necrosis Factor Ligand 1A; CD178 Antigen; ALPS1B; TNLG1A |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48023 Human Tumor necrosis factor ligand superfamily member 6 |
|---|---|
| Gene Symbol | FASLG |
| Gene Full Name | Fas ligand (TNF superfamily, member 6) |
| Background | CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo. |
| Function | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 31 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260). Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. Monoubiquitinated. |
Images (2) Click the Picture to Zoom In
-
ARG65523 anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin) FACS image
Flow Cytometry: FasL transfected L5178Y cells stained with ARG65523 anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin) at 9 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
-
ARG65523 anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin) FACS image
Flow Cytometry: Separation of unstained (black-dashed) and stained (red-filled) FasL transfected L5178Y cells. Cells were stained with ARG65523 anti-CD178 / Fas Ligand antibody [NOK-1] (low endotoxin) at 9 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
