ARG42302
anti-CD169 / Siglec 1 antibody [7-239] (APC)
anti-CD169 / Siglec 1 antibody [7-239] (APC) for Flow cytometry and Human
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [7-239] recognizes CD169 / Siglec 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7-239 |
| Isotype | IgG1 |
| Target Name | CD169 / Siglec 1 |
| Antigen Species | Human |
| Immunogen | Human rhinovirus 14-infected monocyte-derived dendritic cells. |
| Conjugation | APC |
| Alternate Names | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SIGLEC1 |
| Gene Full Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
| Background | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| Calculated MW | 183 kDa |
Images (3) Click the Picture to Zoom In
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension.
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension and ARG62720 anti-CD11c antibody [BU15] (FITC) at 20 µl / 100 µl of peripheral whole blood.
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Separation of Human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed). Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension.
