ARG58198

anti-CD166 antibody

anti-CD166 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD166
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD166
Antigen Species Human
Immunogen Synthetic peptide derived from Human CD166.
Conjugation Un-conjugated
Alternate Names MEMD; CD166 antigen; CD antigen CD166; Activated leukocyte cell adhesion molecule; CD166

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:60
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:40
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Daudi
Observed Size ~ 96 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11658 Mouse ALCAM

GeneID: 214 Human ALCAM

GeneID: 79559 Rat ALCAM

Gene Symbol ALCAM
Gene Full Name activated leukocyte cell adhesion molecule
Background This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. [UniProt]
Calculated MW 65 kDa
PTM Glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG58198 anti-CD166 antibody WB image

    Western blot: Daudi cell lysate stained with ARG58198 anti-CD166 antibody.