ARG65461

anti-CD161 antibody [HP-3G10]

anti-CD161 antibody [HP-3G10] for CyTOF®-candidate,Flow cytometry,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [HP-3G10] recognizes CD161
Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, WB
Specificity The clone HP-3G10 recognizes CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
Host Mouse
Clonality Monoclonal
Clone HP-3G10
Isotype IgG1
Target Name CD161
Antigen Species Human
Immunogen human NK cells
Conjugation Un-conjugated
Alternate Names CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3820 Human KLRB1

Swiss-port # Q12918 Human Killer cell lectin-like receptor subfamily B member 1

Gene Symbol KLRB1
Gene Full Name killer cell lectin-like receptor subfamily B, member 1
Background CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on a majority of NK cells, NKT cells, and e.g. Th17 cells and CD3+ thymocytes. Although rat CD161 has three isoforms (a, b, c), the human CD161 is expressed as one isoform.
Function Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt]
Highlight Related products:
CD161 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM N-glycosylated. Contains sialic acid residues.

Images (2) Click the Picture to Zoom In

  • ARG65461 anti-CD161 antibody [HP-3G10] FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG65461 anti-CD161 antibody [HP-3G10] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG65461 anti-CD161 antibody [HP-3G10] FACS image

    Flow Cytometry: Separation of human CD161 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65461 anti-CD161 antibody [HP-3G10] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase.

Pozo D et al.
J Immunol.,  (2006)

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