ARG42650
anti-CD153 / CD30 Ligand antibody
anti-CD153 / CD30 Ligand antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD153 / CD30 Ligand |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD153 / CD30 Ligand |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to Q63-D234 of Human CD153 / CD30 Ligand. |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor necrosis factor ligand superfamily member 8; CD30L; CD30-L; CD153; CD antigen CD153; CD30LG; CD30 ligand |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P32971 Human Tumor necrosis factor ligand superfamily member 8 |
|---|---|
| Gene Symbol | TNFSF8 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 8 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| Function | Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 26 kDa |
Images (2) Click the Picture to Zoom In
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ARG42650 anti-CD153 / CD30 Ligand antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG42650 anti-CD153 / CD30 Ligand antibody at 1 µg/ml dilution.
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ARG42650 anti-CD153 / CD30 Ligand antibody FACS image
Flow Cytometry: U937 cells were blocked with 10% normal goat serum and then stained with ARG42650 anti-CD153 / CD30 Ligand antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
