ARG66230
anti-CD15 antibody [MMA]
anti-CD15 antibody [MMA] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [MMA] recognizes CD15 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P |
| Specificity | This antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin's lymphoma. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MMA |
| Isotype | IgM |
| Target Name | CD15 |
| Antigen Species | Human |
| Immunogen | U937 histiocytic lymphoma cells. |
| Conjugation | Un-conjugated |
| Alternate Names | LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen retrieval: Heat mediated. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by precipitation and chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FUT4 |
| Gene Full Name | fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) |
| Background | The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009] |
| Function | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt] |
| Highlight | Related products: CD15 antibodies; CD15 ELISA Kits; CD15 Duos / Panels; Anti-Mouse IgM secondary antibodies; Related news: Lymphoma |
| Calculated MW | 59 kDa |
Images (3) Click the Picture to Zoom In
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ARG66230 anti-CD15 antibody [MMA] FACS image
Flow Cytometry: Human peripheral blood stained with ARG66230 anti-CD15 antibody [MMA].
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ARG66230 anti-CD15 antibody [MMA] IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG66230 anti-CD15 antibody [MMA] at 10 µg/ml dilution.
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ARG66230 anti-CD15 antibody [MMA] IHC-P image
Immunohistochemistry: Formalin / PFA-fixed and paraffin-embedded human Hodgkins lymphoma stained with ARG66230 anti-CD15 antibody [MMA].
