ARG66252
anti-CD138 / Syndecan 1 antibody [SQab1741]
anti-CD138 / Syndecan 1 antibody [SQab1741] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1741] recognizes CD138 / Syndecan 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1741 |
| Isotype | IgG |
| Target Name | CD138 / Syndecan 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 200-300 of Human CD138. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD138; syndecan; CD138; SDC; Syndecan-1; SYND1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SDC1 |
| Gene Full Name | syndecan 1 |
| Background | The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008] |
| Function | Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. [UniProt] |
| Highlight | Related products: CD138 antibodies; CD138 ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 32 kDa |
| PTM | Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66252 anti-CD138 / Syndecan 1 antibody [SQab1741] FACS image
Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66252 anti-CD138 / Syndecan 1 antibody [SQab1741] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (Black) was used as a control.
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ARG66252 anti-CD138 / Syndecan 1 antibody [SQab1741] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Liver tissue stained with ARG66252 anti-CD138 / Syndecan 1 antibody [SQab1741] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
