ARG52327
anti-CD107a / LAMP1 antibody [5H6]
anti-CD107a / LAMP1 antibody [5H6] for ICC/IF,Western blot and Human,Bovine
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [5H6] recognizes CD107a / LAMP1 |
|---|---|
| Tested Reactivity | Hu, Bov |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H6 |
| Isotype | IgG1 |
| Target Name | CD107a / LAMP1 |
| Antigen Species | Human |
| Immunogen | Recombinant full length human LAMP1 expressed in and purified from E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~100k LAMP1 protein * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS and 10 mM Sodium azide |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11279 Human Lysosome-associated membrane glycoprotein 1 Swiss-port # Q05204 Bovine Lysosome-associated membrane glycoprotein 1 |
|---|---|
| Gene Symbol | LAMP1 |
| Gene Full Name | lysosomal-associated membrane protein 1 |
| Background | Lysosomal Associated Membrane Protein1 (LAMP1) is a protein that is localized primarily in lysosomes but may also be present on late endosomes and the plasma membrane. LAMP1 antibodies are therefore widely used as lysosome markers. It has recently been suggested that lysosomes are activated in microglia in the progression of multiple system atrophy (MSA) and thus play a key role in its pathology (Makioka et al., 2012). |
| Highlight | Related Antibody Duos and Panels: ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1) Related products: LAMP1 antibodies; LAMP1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related poster download: Organelle Markers & Loading Control |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody |
| Calculated MW | 45 kDa |
| PTM | O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells. |
Images (3) Click the Picture to Zoom In
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] at 1:5000 dilution.
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] ICC/IF image
Immunofluorescence: HeLa cells were treated with 50 µM of chloroquine, an inhibitor of autophagy, for 16 hours prior to staining. Cells stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] (red) at 1:500 dilution, and costained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
Clone 5H6 reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] WB image
Western blot: Cells were untreated or treated with 50 µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours. HeLa, HeLa + CQ, NIH/3T3 and NIH/3T3 + CQ (left to right) cell lysates stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] (green) at 1:10000 dilution. The smeared band between 75-120 kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the Human cells, this antibody does not recognize the rodent LAMP1 homologue.
The same blot was stained with ARG10757 anti-Hsp 60 antibody (red) at 1:20000 dilution.
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Review for anti-CD107a / LAMP1 antibody [5H6]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
