ARG23943

anti-CD104 / Integrin beta 4 antibody [M126]

anti-CD104 / Integrin beta 4 antibody [M126] for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [M126] recognizes CD104 / Integrin beta 4
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone M126
Isotype IgG1
Target Name CD104 / Integrin beta 4
Antigen Species Human
Immunogen Recombinant protein around the cytoplasmic region of Human CD104 / Integrin beta 4.
Conjugation Un-conjugated
Alternate Names Integrin beta-4; GP150; CD antigen CD104; CD104

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:250
WB1:1000
Application Note WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 50% Glycerol and 1 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3691 Human ITGB4

Swiss-port # P16144 Human Integrin beta-4

Gene Symbol ITGB4
Gene Full Name integrin, beta 4
Background Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes. [UniProt]
Calculated MW 202 kDa
PTM Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secundary association with tertaspanins. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG23943 anti-CD104 / Integrin beta 4 antibody [M126] ICC/IF image

    Immunofluorescence: A431 cells untreated (left) or treated with pervanadate (right). Cells were stained with ARG23943 anti-CD104 / Integrin beta 4 antibody [M126].

  • ARG23943 anti-CD104 / Integrin beta 4 antibody [M126] WB image

    Western blot: A431 cells treated with serum starved overnight (left) and treated with pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23943 anti-CD104 / Integrin beta 4 antibody [M126].