ARG55069
anti-CBF beta antibody
anti-CBF beta antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CBF beta |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CBF beta |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 61-90 (Center) of Human CBF beta. |
| Conjugation | Un-conjugated |
| Alternate Names | PEBP2-beta; Polyomavirus enhancer-binding protein 2 beta subunit; CBF-beta; SL3-3 enhancer factor 1 subunit beta; PEA2-beta; PEBP2B; SL3/AKV core-binding factor beta subunit; Core-binding factor subunit beta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CBFB |
| Gene Full Name | core-binding factor, beta subunit |
| Background | The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Gene Regulation antibody |
| Calculated MW | 22 kDa |
Images (3) Click the Picture to Zoom In
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ARG55069 anti-CBF beta antibody ICC/IF image
Immunofluorescence: 293 cells stained with ARG55069 anti-CBF beta antibody.
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ARG55069 anti-CBF beta antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG55069 anti-CBF beta antibody.
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ARG55069 anti-CBF beta antibody FACS image
Flow Cytometry: HL-60 cells stained with ARG55069 anti-CBF beta antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
