ARG64062
anti-CARD15 / NOD2 antibody
anti-CARD15 / NOD2 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CARD15 / NOD2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CARD15 / NOD2 |
| Antigen Species | Human |
| Immunogen | KFRFTDRERHCSPTD |
| Conjugation | Un-conjugated |
| Alternate Names | Inflammatory bowel disease protein 1; PSORAS1; BLAU; CARD15; CD; CLR16.3; NLRC2; Caspase recruitment domain-containing protein 15; IBD1; ACUG; Nucleotide-binding oligomerization domain-containing protein 2; NOD2B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9HC29 Human Nucleotide-binding oligomerization domain-containing protein 2 |
|---|---|
| Background | This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 115 kDa |
| PTM | Polyubiquitinated following MDP stimulation, leading to proteasome-mediated degradation (PubMed:23019338). |
Images (1) Click the Picture to Zoom In
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ARG64062 anti-CARD15 / NOD2 antibody WB image
Western Blot: Human Peripheral Blood Mononucleocyte lysate (35 µg protein in RIPA buffer) stained with ARG64062 anti-NOD2 antibody at 0.1 µg/ml dilution.
