ARG55885
anti-Bmi 1 antibody
anti-Bmi 1 antibody for ICC/IF,Western blot and Human,Mouse
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bmi 1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bmi 1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 281-314 (C-terminus) of Human Bmi 1. |
| Conjugation | Un-conjugated |
| Alternate Names | Polycomb complex protein BMI-1; RING finger protein 51; Polycomb group RING finger protein 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | COMMD3-BMI1 |
|---|---|
| Gene Full Name | COMMD3-BMI1 readthrough |
| Background | This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011] |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. |
| Calculated MW | 37 kDa |
| PTM | Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. |
Images (2) Click the Picture to Zoom In
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ARG55885 anti-Bmi 1 antibody WB image
Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55885 anti-Bmi 1 antibody at 1:1000 dilution.
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ARG55885 anti-Bmi 1 antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG55885 anti-Bmi 1 antibody at 1:25 dilution (green). Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (red) at 1:100 dilution.
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Review for anti-Bmi 1 antibody
Application：WB
Sample：HeLa and A549
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.
WB / Human
