ARG65614
anti-Beclin 1 antibody
anti-Beclin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Beclin 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Beclin 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of human BECN1 |
| Conjugation | Un-conjugated |
| Alternate Names | BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 0.9 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BECN1 |
| Gene Full Name | beclin 1, autophagy related |
| Background | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. [UniProt] |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus. [UniProt] |
| Highlight | Related products: Beclin 1 antibodies; Beclin 1 Duos / Panels; |
| Research Area | Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody |
| Calculated MW | 52 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG65614 anti-Beclin 1 antibody WB image
Western blot: 20 µg of U87-MG cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:1000 dilution.
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ARG65614 anti-Beclin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/20 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).
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ARG65614 anti-Beclin 1 antibody WB image
Western blot: 40 µg of NIH/3T3 cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:700 dilution.
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ARG65614 anti-Beclin 1 antibody WB image
Western blot: 30 µg of A549, U87-MG, HepG2, Rat brain, and Rat ovary lysates stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.
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ARG65614 anti-Beclin 1 antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.
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ARG65614 anti-Beclin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/70 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).
Customer's Feedback
Excellent
Review for anti-Beclin 1 antibody
Application：WB
Sample：U87-MG
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Beclin 1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Class IIa histone deacetylase inhibition ameliorates acute kidney injury by suppressing renal tubular cell apoptosis and enhancing autophagy and proliferation
WB / Mouse
Role of Autophagy in Acquired Cholesteatoma.
WB / Human
新生大鼠早期感染致脑白质损伤自噬相关蛋白的表达
WB / Rat
