ARG65415

anti-Bcl 2 antibody [Bcl2/100/D5]

anti-Bcl 2 antibody [Bcl2/100/D5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
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Overview

Product Description Mouse Monoclonal antibody [Bcl-2/100/D5] recognizes Bcl 2
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone Bcl2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Host Mouse
Clonality Monoclonal
Clone Bcl2/100/D5
Isotype IgG1
Target Name Bcl 2
Antigen Species Human
Immunogen Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Conjugation Un-conjugated
Alternate Names BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 596 Human BCL2

Swiss-port # P10415 Human Apoptosis regulator Bcl-2

Gene Symbol BCL2
Gene Full Name B-cell CLL/lymphoma 2
Background This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt]
Cellular Localization Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax)
ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid)
Related products:
Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Lymphoma
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW 26 kDa
PTM Phosphoprotein, Ubl conjugation. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] WB image

    Western blot: 40 µg of Jurkat cell lysate stained with ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] at 1:500 dilution.

  • ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] at 10 µg/ml dilution.

Customer's Feedback

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Review for anti-Bcl 2 antibody [Bcl2/100/D5]

Application:WB

Sample:Jurkat cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC