ARG55188

anti-Bcl 2 antibody

anti-Bcl 2 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Bcl 2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bcl 2
Antigen Species Human
Immunogen Synthetic peptide of Human Bcl-2 (Swiss: P10415)
Conjugation Un-conjugated
Alternate Names BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IP1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12043 Mouse BCL2

GeneID: 24224 Rat BCL2

GeneID: 596 Human BCL2

Gene Symbol BCL2
Gene Full Name B-cell CLL/lymphoma 2
Background This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt]
Cellular Localization Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax)
ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid)
Related products:
Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Lymphoma
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW 26 kDa
PTM Phosphoprotein, Ubl conjugation. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG55188 anti-Bcl 2 antibody WB image

    Western blot: 30 μg of Jurkat cell lysate stained with ARG55188 anti-Bcl 2 antibody at 1:1000 dilution.

  • ARG55188 anti-Bcl 2 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55188 anti-Bcl 2 antibody (red) at 1:100 dilution. DAPI (blue) for nuclear staining.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Bcl 2 antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Bcl 2 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Bcl 2 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Transcutaneous electrical acupoint stimulation alleviates cerebral ischemic injury through the TLR4/MyD88/NF-κ B pathway

WB / Rat

Front Cell Neurosc. et al.
Front Cell Neurosci.,  (2024)

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Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes

WB / Human

Limin Zhang et al.
Heliyon.,  (2024)

publication_link

 

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Probucol attenuates high glucose-induced Müller cell damage through enhancing the Nrf2/p62 signaling pathway

WB / Human

Yu-Fan Zhou et al.
Int Ophthalmol.,  (2023)

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Dapagliflozin protects against doxorubicin-induced nephrotoxicity associated with nitric oxide pathway-A translational study

WB / Rat

Wei-Ting Chang et al.
Free Radic Biol Med.,  (2023)

publication_link

 

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Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage

WB / Rat

Xuecheng Zheng et al.
Chin Med J (Engl).,  (2023)

publication_link

 

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Bone targeted miRNA delivery system for miR-34a with enhanced anti-tumor efficacy to bone-associated metastatic breast cancer

WB / Human

Tian-Yan Han et al.
Int J Pharm.,  (2023)

publication_link

 

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Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells

WB / Human

Ying Liang et al.
Gynecol Endocrinol.,  (2023)

publication_link

 

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Dapagliflozin-entresto protected kidney from renal hypertension via downregulating cell-stress signaling and upregulating SIRT1/PGC-1α/Mfn2-medicated mitochondrial homeostasis

WB / Rat

Sheung-Fat Ko et al.
Exp Biol Med (Maywood),  (2023)

publication_link

 

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Ivabradine could not decrease mitral regurgitation triggered atrial fibrosis and fibrillation compared with carvedilol

WB / Rat

Wei-Chieh Lee et al.
ESC Heart Fail.,  (2023)

publication_link

 

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Transpulmonary Expression of Exosomal microRNAs in Idiopathic and Congenital Heart Disease-Related Pulmonary Arterial Hypertension

WB / Human

Wei-Ting Chang et al.
J Am Heart Assoc,  (2023)

publication_link

 

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Astaxanthin alleviates fine particulate matter (PM2.5)-induced lung injury in rats by suppressing ferroptosis and apoptosis

WB / Rat

Bowen Yin et al.
Food & Function.,  (2023)

publication_link

 

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Orlistat Resensitizes Sorafenib-Resistance in Hepatocellular Carcinoma Cells through Modulating Metabolism

WB / Human

Pei-Wei Shueng et al.
International Journal of Molecular Sciences,  (2022)

publication_link

 

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Deletion of MicroRNA-21 Impairs Neovascularization Following Limb Ischemia: From Bedside to Bench

WB / Mouse

Wei-Ting Chang et al.
Front Cardiovasc Med.,  (2022)

publication_link

 

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Naringin Interferes Doxorubicin-Induced Myocardial Injury by Promoting the Expression of ECHS1

ICC/IF / Rat

Zirui Zhao et al.
Front Pharmacol.,  (2022)

publication_link

 

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Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

WB / Human

Tzu-Chun Kan et al.
Pharmaceutics.,  (2022)

publication_link

 

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Dapagliflozin Improves Cardiac Hemodynamics and Mitigates Arrhythmogenesis in Mitral Regurgitation-Induced Myocardial Dysfunction.

WB / Rat

Yu-Wen Lin et al.
J Am Heart Assoc.,  (2021)

publication_link

 

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Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

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Xenogeneic and Allogeneic Mesenchymal Stem Cells Effectively Protect the Lung Against Ischemia-reperfusion Injury Through Downregulating the Inflammatory, Oxidative Stress, and Autophagic Signaling Pathways in Rat.

WB / Human

Kun-Chen Lin et al.
Cell Transplant.,  (2020)

publication_link

 

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miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI

WB / Human

Xue Luo et al.
Biosci Rep.,  (2020)

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Possible Involvement of PTEN Signaling Pathway in the Anti-apoptotic Effect of Electroacupuncture Following Ischemic Stroke in Rats

WB / Rat

Xing Ying et al.
Cell Mol Neurobiol.,  (2018)

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Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.

WB / Rat

Xing Ying et al.
J Mol Neurosci.,  (2018)

publication_link

 

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

WB / Mouse

Xuan Wang et al.
Oncol Lett.,  (2018)

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