ARG55935
anti-Bcl 2 antibody [124]
anti-Bcl 2 antibody [124] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [124] recognizes Bcl 2 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 124 |
| Isotype | IgG1, kappa |
| Target Name | Bcl 2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 41-54 of Human Bcl-2 protein (GAAPAPGIFSSQPG-C). |
| Conjugation | Un-conjugated |
| Alternate Names | BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 1 mM EDTA buffer (pH 7.5-8.5) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCL2 |
| Gene Full Name | B-cell CLL/lymphoma 2 |
| Background | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt] |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax) ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid) Related products: Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| Calculated MW | 26 kDa |
| PTM | Phosphoprotein, Ubl conjugation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG55935 anti-Bcl 2 antibody [124] WB image
Western blot: 30 µg of MCF-7 cell lysate stained with ARG55935 anti-Bcl 2 antibody [124] at 1:200 dilution.
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ARG55935 anti-Bcl 2 antibody [124] FACS image
Flow Cytometry: Jurkat cells stained with ARG55935 anti-Bcl 2 antibody [124] (red) and isotype control antibody (green).
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ARG55935 anti-Bcl 2 antibody [124] IHC-P image
Immunohistochemistry: Human non-Hodgkin's lymphoma stained with ARG55935 anti-Bcl 2 antibody [124]. Note nuclear membrane and cytoplasmic staining.
Customer's Feedback
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Review for anti-Bcl 2 antibody [124]
Application：WB
Sample：MCF-7
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
