ARG65612
anti-Bax antibody
anti-Bax antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Publication5
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bax |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bax |
| Antigen Species | Human |
| Immunogen | Fusion protein of human BAX |
| Conjugation | Un-conjugated |
| Alternate Names | Bcl-2-like protein 4; Bcl2-L-4; BCL2L4; Apoptosis regulator BAX |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 1.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BAX |
| Gene Full Name | BCL2-associated X protein |
| Background | Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019] |
| Function | Bax plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30263 Mitochondrial fission Antibody Duo (Drp1, BAX) ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax) Related products: Bax antibodies; Bax Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Understanding Your Cells: Choose the right markers |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody |
| Calculated MW | 21 kDa |
Images (5) Click the Picture to Zoom In
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ARG65612 anti-Bax antibody WB image
Western blot: 30 µg of MCF-7, HCT116, U87-MG, and HepG2 cell line lysates stained with ARG65612 anti-Bax antibody at 1:500 dilution.
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ARG65612 anti-Bax antibody ICC/IF image
Immunocytochemistry: HeLa cells fixed with Methanol / Acetone 1:1 ratio (-20°C for 20 min) and blocked by 3% BSA in PBS (RT for 1 hour). The cells stained with ARG65612 anti-Bax antibody at 1:20 dilution (RT for 1 hour).
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ARG65612 anti-Bax antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human esophagus cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG65612 anti-Bax antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human lung cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG65612 anti-Bax antibody WB image
Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG65612 anti-Bax antibody at 1:3000 dilution.
Customer's Feedback
Excellent
Review for anti-Bax antibody
Application：WB
Sample：U87
Sample Loading Amount：20 µl
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Bax antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：Methanol / Acetone 1:1 ratio
Fixation Time：20 min
Fixation Temperature：-20 ºC
Antigen Retrieval Buffer：N/A
Antigen Retrieval Time：N/A
Antigen Retrieval Temperature：N/A ºC
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：1 hour
Primary Antibody Incubation Temperature：RT ºC
Specific References
Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions
WB / Human
Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers
WB / Human
Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling
WB / Human
Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.
WB / Rat
A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis
WB / Mouse
