ARG58336

anti-BNIP3 antibody

anti-BNIP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes BNIP3
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BNIP3
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-140 of Human BNIP3 (NP_004043.3).
Conjugation Un-conjugated
Alternate Names BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; NIP3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A375
Observed Size ~ 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12176 Mouse BNIP3

GeneID: 664 Human BNIP3

Swiss-port # O55003 Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Swiss-port # Q12983 Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Gene Symbol BNIP3
Gene Full Name BCL2/adenovirus E1B 19kDa interacting protein 3
Background This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]
Function Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. [UniProt]
Cellular Localization Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein. [UniProt]
Highlight Related products:
BNIP3 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Hypoxia-induced transcription, histone demethylases are involved
Calculated MW 28 kDa

Images (3) Click the Picture to Zoom In

  • ARG58336 anti-BNIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat stomach stained with ARG58336 anti-BNIP3 antibody at 1:100 dilution.

  • ARG58336 anti-BNIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse stomach stained with ARG58336 anti-BNIP3 antibody at 1:100 dilution.

  • ARG58336 anti-BNIP3 antibody WB image

    Western blot: 25 µg of A375 cell lysate stained with ARG58336 anti-BNIP3 antibody at 1:1000 dilution.

    The ~ 60 kDa signal might be a homodimer form of BNIP3.