ARG58336
anti-BNIP3 antibody
anti-BNIP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BNIP3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BNIP3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-140 of Human BNIP3 (NP_004043.3). |
| Conjugation | Un-conjugated |
| Alternate Names | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; NIP3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A375 | ||||||
| Observed Size | ~ 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O55003 Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 Swiss-port # Q12983 Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
|---|---|
| Gene Symbol | BNIP3 |
| Gene Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3 |
| Background | This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014] |
| Function | Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. [UniProt] |
| Cellular Localization | Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein. [UniProt] |
| Highlight | Related products: BNIP3 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Hypoxia-induced transcription, histone demethylases are involved |
| Calculated MW | 28 kDa |
Images (3) Click the Picture to Zoom In
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ARG58336 anti-BNIP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat stomach stained with ARG58336 anti-BNIP3 antibody at 1:100 dilution.
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ARG58336 anti-BNIP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse stomach stained with ARG58336 anti-BNIP3 antibody at 1:100 dilution.
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ARG58336 anti-BNIP3 antibody WB image
Western blot: 25 µg of A375 cell lysate stained with ARG58336 anti-BNIP3 antibody at 1:1000 dilution.
The ~ 60 kDa signal might be a homodimer form of BNIP3.
