ARG65673
anti-BDNF antibody
anti-BDNF antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Publication3
Overview
| Product Description | Goat Polyclonal antibody recognizes BDNF |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | BDNF |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human BDNF (ETKCNPMGYTKE) |
| Conjugation | Un-conjugated |
| Alternate Names | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: WB was tested by the publication (dio: 10.21203/rs.3.rs-929317/v1) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BDNF |
| Gene Full Name | brain-derived neurotrophic factor |
| Background | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| Function | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| Highlight | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 28 kDa |
| PTM | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). |
Images (6) Click the Picture to Zoom In
-
ARG65673 anti-BDNF antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG65673 anti-BDNF antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
-
ARG65673 anti-BDNF antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Mouse sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.
-
ARG65673 anti-BDNF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65673 anti-BDNF antibody at 3.75 µg/ml dilution followed by AP-staining.
-
ARG65673 anti-BDNF antibody IHC image
Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B) stained with ARG65673 anti-BDNF antibody (red) at 5 ug/ml dilution. CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98°C.
-
ARG65673 anti-BDNF antibody IHC image
Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex stained with ARG65673 anti-BDNF antibody at 5 ug/ml dilution. Antigen retrieval with citrate buffer pH 6 at 95-98°C.
-
ARG65673 anti-BDNF antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Mouse injured sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.
Specific References
G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation
WB / Mouse
Neuroprotective Effects of Estrogen Through BDNF-Transient Receptor Potential Channels 6 Signaling Pathway in the Hippocampus in a Rat Model of Perimenopausal Depression
function /
p53 Knock Out Mice Enhances Anxiety- and Depression-Like Behaviors through an Increase of Glutamate, Calcium, and Cytokine-Mediated Cell Death Signals
WB / Mouse
