ARG43129
anti-BCL9L antibody
anti-BCL9L antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BCL9L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BCL9L |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E397-A666 of Human BCL9L. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein BCL9-2; BCL9-2; B-cell CLL/lymphoma 9-like protein; DLNB11; BCL9-like protein; B-cell lymphoma 9-like protein |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q67FY2 Mouse B-cell CLL/lymphoma 9-like protein Swiss-port # Q86UU0 Human B-cell CLL/lymphoma 9-like protein |
|---|---|
| Gene Symbol | BCL9L |
| Gene Full Name | B-cell CLL/lymphoma 9-like |
| Function | Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 157 kDa |
Images (9) Click the Picture to Zoom In
-
ARG43129 anti-BCL9L antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG43129 anti-BCL9L antibody (green) at 2 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG43129 anti-BCL9L antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG43129 anti-BCL9L antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG43129 anti-BCL9L antibody (green) at 2 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
