ARG42744
anti-B7-H4 antibody
anti-B7-H4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes B7-H4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | B7-H4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to T57-A258 of Human B7-H4. |
| Conjugation | Un-conjugated |
| Alternate Names | B7H4; B7 homolog 4; Protein B7S1; V-set domain-containing T-cell activation inhibitor 1; B7-H4; B7X; T-cell costimulatory molecule B7x; B7h.5; VCTN1; PRO1291; Immune costimulatory protein B7-H4; B7S1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7Z7D3 Human V-set domain-containing T-cell activation inhibitor 1 |
|---|---|
| Gene Symbol | VTCN1 |
| Gene Full Name | V-set domain containing T cell activation inhibitor 1 |
| Background | This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Expressed at the cell surface. A soluble form has also been detected. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | N-glycosylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42744 anti-B7-H4 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG42744 anti-B7-H4 antibody (green) at 2 µg/ml dilutioin, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42744 anti-B7-H4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42744 anti-B7-H4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42744 anti-B7-H4 antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG42744 anti-B7-H4 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
