ARG10648
anti-Aquaporin 3 antibody
anti-Aquaporin 3 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Aquaporin 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Aquaporin 3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human Aquaporin 3. (EENVKLAHVKHKEQI) |
| Conjugation | Un-conjugated |
| Alternate Names | Aquaporin-3; GIL; Aquaglyceroporin-3; AQP-3 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.025% Sodium azide and 2.5% BSA. |
| Preservative | 0.025% Sodium azide |
| Stabilizer | 2.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AQP3 |
| Gene Full Name | aquaporin 3 (Gill blood group) |
| Background | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011] |
| Function | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity). [UniProt] |
| Calculated MW | 32 kDa |
Images (7) Click the Picture to Zoom In
-
ARG10648 anti-Aquaporin 3 antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG10648 anti-Aquaporin 3 antibody.
-
ARG10648 anti-Aquaporin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human renal cancer tissue. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG10648 anti-Aquaporin 3 antibody.
-
ARG10648 anti-Aquaporin 3 antibody WB image
Western blot: 1) Rat kidney, 2) Rat lung, 3) Mouse kidney, 4) MDA-MB-453, a human breast cancer line, and 5) SMMC-7221, a human hepatocarcinoma cell line. Lysates stained with ARG10648 anti-Aquaporin 3 antibody.
-
ARG10648 anti-Aquaporin 3 antibody FACS image
Flow Cytometry: HeLa cells were blocked with goat sera and stained with ARG10648 anti-Aquaporin 3 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
-
ARG10648 anti-Aquaporin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG10648 anti-Aquaporin 3 antibody.
-
ARG10648 anti-Aquaporin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG10648 anti-Aquaporin 3 antibody.
-
ARG10648 anti-Aquaporin 3 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat kidney tissue stained with ARG10648 anti-Aquaporin 3 antibody.
Specific References
Pterostilbene Attenuates Particulate Matter-Induced Oxidative Stress, Inflammation and Aging in Keratinocytes
WB / Human
Predicting the effects of radiotherapy based on diffusion kurtosis imaging in a xenograft mouse model of esophageal carcinoma
WB / Human
Improvement of Skin Penetration, Antipollutant Activity and Skin Hydration of 7,3',4'-Trihydroxyisoflavone Cyclodextrin Inclusion Complex.
WB / Human
