ARG22360
anti-Aquaporin 2 antibody
anti-Aquaporin 2 antibody for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Aquaporin 2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, WB |
| Specificity | Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Aquaporin 2 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around the C-terminus of Rat Aquaporin 2. (N-CLKGLEPDTDWEEREVRRRQ) |
| Conjugation | Un-conjugated |
| Alternate Names | Aquaporin-2; Aquaporin-CD; AQP-2; ADH water channel; Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP-CD; WCH-CD |
Application Instructions
| Application Suggestion |
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| Application Note | WB: 0.5 µg/ml of this antibody was sufficient for detection of aquaporin 2 in 10 µg of Rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:HRP as the secondary antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Aqp2 |
| Gene Full Name | aquaporin 2 |
| Background | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008] |
| Function | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. [UniProt] |
| Cellular Localization | Apical cell membrane, Basolateral Cell Membrane, Cell membrane, Cytoplasmic Vesicle, Cytoplasmic vesicle membrane, Golgi apparatus |
| Calculated MW | 28.8 kDa (unmodified); 35 - 50 kDa (glycosylated) |
| PTM | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. |
Images (2) Click the Picture to Zoom In
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ARG22360 anti-Aquaporin 2 antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG22360 anti-Aquaporin 2 antibody (green) at 1:200 dilution.
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ARG22360 anti-Aquaporin 2 antibody WB image
Western blot: Rat kidney inner medullary homogenates stained with ARG22360 anti-Aquaporin 2 antibody at 1:2000 dilution. Showing glycosylated and non-glycosylated bands.
Customer's Feedback
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Review for anti-Aquaporin 2 antibody
Application：WB
Sample：Mouse heart
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
