ARG43659
anti-AlaRS / Alanyl tRNA Synthetase antibody
anti-AlaRS / Alanyl tRNA Synthetase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AlaRS / Alanyl tRNA Synthetase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AlaRS / Alanyl tRNA Synthetase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within a.a. 1-150 of Human Alanyl-tRNA Synthetase. |
| Conjugation | Un-conjugated |
| Protein Full Name | Alanine--tRNA ligase, cytoplasmic |
| Alternate Names | Renal carcinoma antigen NY-REN-42; CMT2N; EIEE29; AlaRS; EC 6.1.1.7; Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | WB: HeLa cell lysates; IHC: Human lung cancer tissue. | ||||||||
| Observed Size | 110-120 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 0.01% Thiomersal and 50% Glycerol. |
| Preservative | 0.01% Thiomersal |
| Stabilizer | 50% Glycerol |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AARS |
| Gene Full Name | alanyl-tRNA synthetase |
| Background | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. [UniProt] |
| Cellular Localization | Cytoplasm [UniProt] |
| Calculated MW | 107 kDa |
| PTM | ISGylated. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Lung tissue stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution.
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody ICC/IF image
Immunofluorescence: 293T cells were stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100.
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image WB image
Western blot: HeLa cell lysates stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:3000 dilution
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat Spleen tissue stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution.
