ARG66382
anti-Actin antibody [SQab18109]
anti-Actin antibody [SQab18109] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and African green monkey,Bovine,Dog,Human,Mouse,Pig,Rat,Zebrafish
Publication5
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab18109] recognizes Actin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, AGMK, Bov, Dog, Pig, Zfsh |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab18109 |
| Isotype | IgG |
| Target Name | Actin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human Actin. |
| Conjugation | Un-conjugated |
| Alternate Names | CFTDM; MPFD; CFTD; ASMA; NEM1; NEM2; NEM3; Alpha-actin-1; ACTA; CFTD1; Actin, alpha skeletal muscle |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACTA1 |
| Gene Full Name | actin, alpha 1, skeletal muscle |
| Background | Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton. |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG66382 anti-Actin antibody [SQab18109] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colonic adenocarcinoma stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
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ARG66382 anti-Actin antibody [SQab18109] WB image
Western blot: 10 µg of Jurkat, HeLa, 293T, A431 and A375 cell lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.
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ARG66382 anti-Actin antibody [SQab18109] WB image
Western blot: 10 µg of MDCK, MDBK, Pig brain, Pig heart, Pig kidney, Zebrafish and Cos-7 lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.
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ARG66382 anti-Actin antibody [SQab18109] WB image
Western blot: 10 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain and Rat liver lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.
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ARG66382 anti-Actin antibody [SQab18109] WB image
Western blot: Human keratinocytes stained with ARG66382 anti-Actin antibody [SQab18109].
From Jia-Wei Shen et al. Int J Mol Sci (2022), doi: 10.3390/ijms232314623, Fig. 9.
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ARG66382 anti-Actin antibody [SQab18109] WB image
Western blot: Human keratinocytes stained with ARG66382 anti-Actin antibody [SQab18109].
From Jia-Wei Shen et al. Int J Mol Sci (2022), doi: 10.3390/ijms232314623, Fig. 9.
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