ARG62520

anti-ATP2B1 / PMCA1 antibody [5F10]

anti-ATP2B1 / PMCA1 antibody [5F10] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Inhibition assay,Western blot and Human,Mouse,Rat,Amphibians,Bovine,Cat,Chicken,Dog,Hamster,Rabbit,Sheep

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [5F10] recognizes ATP2B1 / PMCA1
Tested Reactivity Hu, Ms, Rat, Amph, Bov, Cat, Chk, Dog, Hm, Rb, Sheep
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Inhib, WB
Host Mouse
Clonality Monoclonal
Clone 5F10
Isotype IgG2a
Target Name ATP2B1 / PMCA1
Antigen Species Human
Immunogen Full length native protein (purified) corresponding to Human Calcium Pump pan PMCA ATPase. Purified human erythrocyte calcium ATPase.
Conjugation Un-conjugated
Alternate Names PMCA1; PMCA1kb; EC 3.6.3.8; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium pump isoform 1; Plasma membrane calcium-transporting ATPase 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:20 - 1:100
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:500
IPAssay-dependent
InhibAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009543 Rabbit ATP2B1

GeneID: 29598 Rat ATP2B1

GeneID: 490 Human ATP2B1

Gene Symbol ATP2B1
Gene Full Name ATPase, Ca++ transporting, plasma membrane 1
Background The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. [UniProt]
Cellular Localization Cell membrane
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 135 kDa

Images (1) Click the Picture to Zoom In

  • ARG62520 anti-ATP2B1 / PMCA1 antibody [5F10] ICC/IF image

    Immunofluorescence: BHK-21 cells were fixed with 4% PFA for 10 min and permeabilized in 0.1% saponin/PBS. Cells were stained with ARG62520 anti-ATP2B1 / PMCA1 antibody [5F10].

Clone References

Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea.

WB / Cow

Wright MF et al.
Biol Reprod.,  (2014)

publication_link

 

hr_line

Expression levels of the ABCG2 multidrug transporter in human erythrocytes correspond to pharmacologically relevant genetic variations.

FACS / Human

Kasza I et al.
PLoS One.,  (2012)

publication_link

 

hr_line

Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis.

ICC/IF / Human

Zanni G et al.
Proc Natl Acad Sci U S A.,  (2012)

publication_link

 

hr_line

Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.

WB / Human

Curry MC et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.

IHC / Rat

Chen Q et al.
Eur J Neurosci.,  (2012)

publication_link

 

hr_line

FATP4 contributes as an enzyme to the basal and insulin-mediated fatty acid uptake of C₂C₁₂ muscle cells.

WB / Mouse

Digel M et al.
Am J Physiol Endocrinol Metab.,  (2011)

publication_link

 

hr_line

Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells.

WB / Bovine

Lock JT et al.
Am J Physiol Heart Circ Physiol.,  (2011)

publication_link

 

hr_line

Plasma membrane calcium-ATPase isoform four distribution changes during corneal epithelial wound healing.

WB / Rabbit

Talarico EF Jr et al.
Mol Vis.,  (2010)

publication_link

 

hr_line

Sodium-calcium exchanger and lipid rafts in pig coronary artery smooth muscle.

WB / Pig

Kuszczak I et al.
Biochim Biophys Acta.,  (2011)

publication_link

 

hr_line

Upregulation of Na+/Ca2+ exchanger and TRPC6 contributes to abnormal Ca2+ homeostasis in arterial smooth muscle cells from Milan hypertensive rats.

WB / Rat

Zulian A et al.
Am J Physiol Heart Circ Physiol.,  (2010)

publication_link

 

hr_line

Tamoxifen inhibits TRPV6 activity via estrogen receptor-independent pathways in TRPV6-expressing MCF-7 breast cancer cells.

ICC/IF / Human

Bolanz KA et al.
Mol Cancer Res.,  (2009)

publication_link

 

hr_line

Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation.

WB / Amphibian

El-Jouni W et al.
Dev Biol.,  (2008)

publication_link

 

hr_line

Syntrophin mutation associated with long QT syndrome through activation of the nNOS-SCN5A macromolecular complex.

WB / Human, Mouse

Ueda K et al.
Proc Natl Acad Sci U S A.,  (2008)

publication_link

 

hr_line

ARL4D recruits cytohesin-2/ARNO to modulate actin remodeling.

ICC/IF / Human

Li CC et al.
Mol Biol Cell.,  (2007)

publication_link

 

hr_line

Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps.

IHC / Pig

Sepúlveda MR et al.
J Neurochem.,  (2007)

publication_link

 

hr_line

Mutation of delta-sarcoglycan is associated with Ca(2+) -dependent vascular remodeling in the Syrian hamster.

ICC/IF / Syrian Hamster

Lipskaia L et al.
Am J Pathol.,  (2007)

publication_link

 

hr_line

Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles.

ICC/IF / Primates

Hill JK et al.
J Neurosci.,  (2006)

publication_link

 

hr_line

Inhibitory interaction of the 14-3-3{epsilon} protein with isoform 4 of the plasma membrane Ca(2+)-ATPase pump.

IP / Human

Rimessi A et al.
J Biol Chem.,  (2005)

publication_link

 

hr_line

A developmental profile of the levels of calcium pumps in chick cerebellum.

WB / Bird

Sepúlveda MR et al.
J Neurochem.,  (2005)

publication_link

 

hr_line

Ca2+/calmodulin-dependent protein kinase II is an essential mediator in the coordinated regulation of electrocyte Ca2+-ATPase by calmodulin and protein kinase A.

WB / Chordate*

Valverde RH et al.
J Biol Chem.,  (2005)

publication_link

 

hr_line

Different effects of Hg2+ and Cu2+ on mussel (Mytilus galloprovincialis) plasma membrane Ca2+-ATPase: Hg2+ induction of protein expression.

WB / Mollusca

Burlando B et al.
Comp Biochem Physiol C Toxicol Pharmacol.,  (2004)

publication_link

 

hr_line

Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate.

WB / Chordate*

Molina AJ et al.
J Physiol.,  (2004)

publication_link

 

hr_line

Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).

ICC/IF / Amphibian

Krizaj D et al.
J Comp Neurol.,  (2004)

publication_link

 

hr_line

Thyrotropin-releasing hormone receptor processing: role of ubiquitination and proteasomal degradation.

WB / Hamster

Cook LB et al.
Mol Endocrinol.,  (2003)

publication_link

 

hr_line

Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta.

IHC / Cat

Champion EE et al.
Placenta.,  (2003)

publication_link

 

hr_line

Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells.

IP / Mouse

Bruce JI et al.
J Biol Chem.,  (2002)

publication_link

 

hr_line

nNOS in canine lower esophageal sphincter: colocalized with Cav-1 and Ca2+-handling proteins?

IHC / Dog

Daniel EE et al.
Am J Physiol Gastrointest Liver Physiol.,  (2001)

publication_link

 

hr_line

Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?

ELISA / Rat

Antoine S et al.
J Membr Biol.,  (2001)

publication_link

 

hr_line

Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling.

WB / Dog

Darby PJ et al.
Am J Physiol Lung Cell Mol Physiol.,  (2000)

publication_link

 

hr_line

Localization of sequences within the C-terminal domain of the cystic fibrosis transmembrane conductance regulator which impact maturation and stability.

ICC/IF / Hamster

Gentzsch M et al.
J Biol Chem.,  (2001)

publication_link

 

hr_line

Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies.

WB / Primates

Filoteo AG et al.
J Biol Chem.,  (1997)

publication_link

 

hr_line

Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen.

WB / Chicken

Wasserman RH et al.
Histochemistry.,  (1991)

publication_link

 

hr_line