ARG58251
anti-ATP Citrate Lyase phospho (Ser455) antibody
anti-ATP Citrate Lyase phospho (Ser455) antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP Citrate Lyase phospho (Ser455) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP Citrate Lyase |
| Antigen Species | Human |
| Immunogen | Phospho specific peptide around Ser455 of Human ATP Citrate Lyase (NP_001087.2). |
| Conjugation | Un-conjugated |
| Alternate Names | ACL; ATP-citrate synthase; Citrate cleavage enzyme; CLATP; EC 2.3.3.8; pro-S-; ATP-citrate; ATPCL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | NIH/3T3 + insulin | ||||||||
| Observed Size | 120 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACLY |
| Gene Full Name | ATP citrate lyase |
| Background | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] |
| Function | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 121 kDa |
| PTM | ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody WB image
Western blot: 25 µg of NIH/3T3 cells treated by Insulin (100 nM) for 10 min after serum-starvation overnight. The blot was stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:2000 dilution.
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas tissue stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:100 dilution.
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody IP image
Immunoprecipitation: NIH/3T3 cells were treated by Insulin (100 nM) at 37°C for 10 minutes after serum-starvation overnight. 200 µg extracts of NIH/3T3 cells were immunoprecipitated and stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:1000 dilution.
