ARG55117

anti-ARP2 antibody

anti-ARP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ARP2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ARP2
Antigen Species Human
Immunogen Recombinant protein of Human ARP2 (Swiss: P61160)
Conjugation Un-conjugated
Alternate Names Actin-related protein 2; Actin-like protein 2; ARP2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse spleen and 293T

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10097 Human ACTR2

GeneID: 289820 Rat ACTR2

GeneID: 66713 Mouse ACTR2

Gene Symbol ACTR2
Gene Full Name ARP2 actin-related protein 2 homolog (yeast)
Background The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 45 kDa

Images (3) Click the Picture to Zoom In

  • ARG55117 anti-ARP2 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55117 anti-ARP2 antibody.

  • ARG55117 anti-ARP2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG55117 anti-ARP2 antibody at 1:100 dilution.

  • ARG55117 anti-ARP2 antibody WB image

    Western blot: Mouse spleen and 293T cell lysates stained with ARG55117 anti-ARP2 antibody.