ARG41490
anti-APBB1IP / RIAM antibody
anti-APBB1IP / RIAM antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes APBB1IP / RIAM |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APBB1IP / RIAM |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 647-666 of Human APBB1IP / RIAM. (EQDFMSDLMKALQKKRGNVS) |
| Conjugation | Un-conjugated |
| Alternate Names | RARP-1; INAG1; Proline-rich EVH1 ligand 1; RARP1; Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; PREL-1; Rap1-GTP-interacting adapter molecule; Proline-rich protein 73; APBB1-interacting protein 1; Retinoic acid-responsive proline-rich protein 1; PREL1; RIAM |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7Z5R6 Human Amyloid beta A4 precursor protein-binding family B member 1-interacting pr Swiss-port # Q8R5A3 Mouse Amyloid beta A4 precursor protein-binding family B member 1-interacting pr |
|---|---|
| Gene Symbol | APBB1IP |
| Gene Full Name | amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein |
| Function | Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion. [UniProt] |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cell projection, lamellipodium. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases. [UniProt] |
| Calculated MW | 73 kDa |
Images (8) Click the Picture to Zoom In
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ARG41490 anti-APBB1IP / RIAM antibody ICC image
Immunocytochemistry: Jurkat cells stained with ARG41490 anti-APBB1IP / RIAM antibody.
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ARG41490 anti-APBB1IP / RIAM antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG41490 anti-APBB1IP / RIAM antibody (red) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG41490 anti-APBB1IP / RIAM antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41490 anti-APBB1IP / RIAM antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41490 anti-APBB1IP / RIAM antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG41490 anti-APBB1IP / RIAM antibody.
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ARG41490 anti-APBB1IP / RIAM antibody WB image
Western blot: 50 µg of samples under reducing conditions. THP-1, Jurkat, Raji, U937, Rat thymus and Mouse thymus lysates stained with ARG41490 anti-APBB1IP / RIAM antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41490 anti-APBB1IP / RIAM antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG41490 anti-APBB1IP / RIAM antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41490 anti-APBB1IP / RIAM antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lymphaden tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41490 anti-APBB1IP / RIAM antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41490 anti-APBB1IP / RIAM antibody WB image
Western blot: Rat thymus, Rat spleen, Raji and HL-60 cell lysates stained with ARG41490 anti-APBB1IP / RIAM antibody.
