ARG11123
anti-ANK3 / Ankyrin G antibody [2A8]
anti-ANK3 / Ankyrin G antibody [2A8] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [2A8] recognizes ANK3 / Ankyrin G |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2A8 |
| Isotype | IgG1 |
| Target Name | ANK3 / Ankyrin G |
| Antigen Species | Human |
| Immunogen | The C-terminal 398 amino acids of Human ANK3 / Ankyrin G. |
| Conjugation | Un-conjugated |
| Alternate Names | Ankyrin-3; Ankyrin-G; MRT37; ANKYRIN-G; ANK-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANK3 |
| Gene Full Name | ankyrin 3, node of Ranvier (ankyrin G) |
| Background | Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| Function | In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. Note=In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes. Isoform 5: Cytoplasm, cytoskeleton. Golgi apparatus. [UniProt] |
| Calculated MW | 480 kDa |
Images (3) Click the Picture to Zoom In
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ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] ICC/IF image
Immunofluorescence: HEK293 cells stained with ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] (green) at 1:2000 dilution. Hoechst (blue) for nuclear staining.
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ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain cortex tissue stained with ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] (green) at 1:2000 dilution, and co-stained with ARG10732 anti-Neurofilament NF-L antibody antibody (red) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
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ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] WB image
Western blot: NIH/3T3, C6, HEK293, HeLa, SH-SY5Y, Rat brain and Mouse brain lysates stained with ARG11123 anti-ANK3 / Ankyrin G antibody [2A8] at 1:2000 dilution.
Bands at ~ 190 kDa represent Ankyrin G splice variants, higher molecular weight bands at 270 kDa and 480 kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.
