ARG58177
anti-ALP / Alkaline Phosphatase antibody
anti-ALP / Alkaline Phosphatase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALP / Alkaline Phosphatase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALP / Alkaline Phosphatase |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 274-304 of Human ALP / Alkaline Phosphatase. |
| Conjugation | Un-conjugated |
| Alternate Names | Intestinal-type alkaline phosphatase; EC 3.1.3.1; IAP; Intestinal alkaline phosphatase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human placenta |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09923 Human Intestinal-type alkaline phosphatase |
|---|---|
| Gene Symbol | ALPI |
| Gene Full Name | alkaline phosphatase, intestinal |
| Background | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Calculated MW | 57 kDa |
Images (3) Click the Picture to Zoom In
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ARG58177 anti-ALP / Alkaline Phosphatase antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody (green) at 1:100 dilution, 2 hours at RT. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
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ARG58177 anti-ALP / Alkaline Phosphatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody at 1:100 dilution.
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ARG58177 anti-ALP / Alkaline Phosphatase antibody WB image
Western blot: 20 µg of Human placenta lysate stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody at 1:1000 dilution.
