ARG45172
anti-AKR7A2 antibody
anti-AKR7A2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AKR7A2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AKR7A2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human AKR7A2. |
| Conjugation | Un-conjugated |
| Alternate Names | AKR7A2; aldo-keto reductase family 7 member A2; Aflatoxin B1 aldehyde reductase member 2; AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase; AKR7A2; AFAR; AFAR1; AKR7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43488 Human Aflatoxin B1 aldehyde reductase member 2 Swiss-port # Q8CG45 Rat Aflatoxin B1 aldehyde reductase member 2 |
|---|---|
| Gene Symbol | AKR7A2 |
| Gene Full Name | aldo-keto reductase family 7 member A2 |
| Background | The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| Function | Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.. [UniProt] |
| Cellular Localization | Mitochondrion; Golgi apparatus; Cytoplasm. [UniProt] |
| Calculated MW | 39 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG45172 anti-AKR7A2 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45172 anti-AKR7A2 antibody at 1 μg/ml dilution.
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ARG45172 anti-AKR7A2 antibody WB image
Western blot: 293T and Jurkat stained with ARG45172 anti-AKR7A2 antibody at 0.5 μg/ml dilution.
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ARG45172 anti-AKR7A2 antibody FACS image
Flow Cytometry: THP-1 stained with ARG45172 anti-AKR7A2 antibody at 1 µg/10^6 cells dilution.
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ARG45172 anti-AKR7A2 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45172 anti-AKR7A2 antibody at 1 μg/ml dilution.
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ARG45172 anti-AKR7A2 antibody WB image
Western blot: Rat brain and rat kidney stained with ARG45172 anti-AKR7A2 antibody at 0.5 μg/ml dilution.
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ARG45172 anti-AKR7A2 antibody IHC-P image
Immunohistochemistry: Mouse intestine stained with ARG45172 anti-AKR7A2 antibody at 1 μg/ml dilution.
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ARG45172 anti-AKR7A2 antibody WB image
Western blot: Mouse brain and mouse kidney stained with ARG45172 anti-AKR7A2 antibody at 0.5 μg/ml dilution.
