ARG40257
anti-ACY1 / Aminoacylase 1 antibody
anti-ACY1 / Aminoacylase 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACY1 / Aminoacylase 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACY1 / Aminoacylase 1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-408 of Human ACY1 / Aminoacylase 1 (NP_001185824.1). |
| Conjugation | Un-conjugated |
| Alternate Names | ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1D; EC 3.5.1.14; HEL-S-5; Aminoacylase-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A431, Mouse kidney and Rat kidney | ||||||||
| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACY1 |
| Gene Full Name | aminoacylase 1 |
| Background | This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| Function | Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 46 kDa |
Images (4) Click the Picture to Zoom In
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ARG40257 anti-ACY1 / Aminoacylase 1 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG40257 anti-ACY1 / Aminoacylase 1 antibody.
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ARG40257 anti-ACY1 / Aminoacylase 1 antibody WB image
Western blot: 25 µg of A431 cell lysate stained with ARG40257 anti-ACY1 / Aminoacylase 1 antibody at 1:1000 dilution.
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ARG40257 anti-ACY1 / Aminoacylase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophageal cancer stained with ARG40257 anti-ACY1 / Aminoacylase 1 antibody at 1:100 dilution.
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ARG40257 anti-ACY1 / Aminoacylase 1 antibody WB image
Western blot: 25 µg of Rat kidney and Mouse kidney lysates stained with ARG40257 anti-ACY1 / Aminoacylase 1 antibody at 1:1000 dilution.
