ARG44091
anti-ACAT1 antibody
anti-ACAT1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal recognizes ACAT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACAT1 |
| Antigen Species | Human |
| Immunogen | Human ACAT1 recombinant protein (Position: S10-Q404). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACAT1 |
| Gene Full Name | acetyl-CoA acetyltransferase 1 |
| Background | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| Function | Plays a major role in ketone body metabolism. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
Images (8) Click the Picture to Zoom In
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ARG44091 anti-ACAT1 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.
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ARG44091 anti-ACAT1 antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG44091 anti-ACAT1 antibody at 5 μg/ml dilution.
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ARG44091 anti-ACAT1 antibody WB image
Western blot: HepG2, PC-3, A549 and MCF-7 stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.
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ARG44091 anti-ACAT1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG44091 anti-ACAT1 antibody at 1 µg/10^6 cells dilution.
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ARG44091 anti-ACAT1 antibody WB image
Western blot: Rat heart stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.
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ARG44091 anti-ACAT1 antibody WB image
Western blot: Mouse heart stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.
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ARG44091 anti-ACAT1 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.
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ARG44091 anti-ACAT1 antibody IHC-P image
Immunohistochemistry: Human non-small cell lung stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.
