ARG66331
anti-6X His tag antibody [SQab1879]
anti-6X His tag antibody [SQab1879] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1879] recognizes 6X His tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1879 |
| Isotype | IgG |
| Target Name | 6X His tag |
| Antigen Species | Others |
| Immunogen | KLH-conjugated HHHHHH (6X His). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Controls and Markers antibody; Tag Internal Control antibody |
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Images (6) Click the Picture to Zoom In
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ARG66331 anti-6X His tag antibody [SQab1879] ICC/IF image
Immunofluorescence: 293 cells trasnfected with N-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:10,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66331 anti-6X His tag antibody [SQab1879] FACS image
Flow Cytometry: 293 cells transfected with N-terminal (blue) and C-terminal (red) 6X His tagged gene. The cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66331 anti-6X His tag antibody [SQab1879] WB image
Western blot: 20 µg of 1) fusion protein with N-terminal 6X His tag, 2) fusion protein with C-terminal 6X His tag, 3) Multi-tag fusion protein containing 6X His tag, 4) 293 cells lysate-transfected with N-terminal 6X His tagged gene, 5) 293 cells lysate-transfected with C-terminal 6X His tagged gene, 6) fusion protein with N-terminal 6X His tag, 7) fusion protein with C-terminal 6X His tag, and 8) untransfected 293 cell lysate stained with ARG66331 anti-6X His tag antibody [SQab1879] at 1:2000 dilution.
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ARG66331 anti-6X His tag antibody [SQab1879] IP image
Immunoprecipitation: 0.05 mg of 293 whole cells lysate transfected with N-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, and 3) 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 10 µg (input).
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ARG66331 anti-6X His tag antibody [SQab1879] ICC/IF image
Immunofluorescence: 293 cells trasnfected with C-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66331 anti-6X His tag antibody [SQab1879] IP image
Immunoprecipitation: 0.2 mg of 293 whole cells lysate transfected with C-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, and 3) 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 10 µg (input).
