ARG54492

anti-6X His tag antibody (HRP)

anti-6X His tag antibody (HRP) for ELISA,ICC/IF,Immunohistochemistry,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description HRP-conjugated Rabbit Polyclonal antibody recognizes 6X His tag
Tested Reactivity Other
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name 6X His tag
Antigen Species Others
Immunogen 6-His (HHHHHH) coupled to KLH
Conjugation HRP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:10000 - 1:100000
ICC/IF1:200 - 1:500
IHC1:200 - 1:500
WB1:1000 - 1:30000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 50mM HEPES (pH 7.1), 0.1M NaCl, 0.2% BSA and 0.1% Pro-Clean 400
Preservative 0.1% Pro-Clean 400
Stabilizer 0.2% BSA
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody

Images (1) Click the Picture to Zoom In

  • ARG54492 anti-6X His tag antibody (HRP) WB image

    Western blot: 200, 100, and 50 ng of E. coli lysates containing 6X His tagged fusion protein. The blots were stained with ARG54492 anti-6X His tag antibody (HRP) at 1:25000 dilution.