ARG54490
anti-6X His tag antibody (HRP)
anti-6X His tag antibody (HRP) for ELISA,ICC/IF,Immunohistochemistry,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody
Overview
| Product Description | HRP-conjugated Goat Polyclonal antibody recognizes 6X His tag |
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| Tested Reactivity | Other |
| Tested Application | ELISA, ICC/IF, IHC, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 6X His tag |
| Antigen Species | Others |
| Immunogen | 6-His (HHHHHH) coupled to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support then conjugated to horseradish peroxidase (HRP). NOTE: This antibody does not detect all N-terminal 6-His tagged proteins. |
| Conjugation | HRP |
Application Instructions
| Application Note | ELISA: 1:10,000 - 1:100,000 Western blot: Colorimetric: 1:1,000 - 1:10,000 Chemiluminescent: 1:1,000 - 1:30,000 Immunohistochemistry: not recommended Immunocytochemistry: 1:200 - 1:500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
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| Buffer | PBS, 0.2% BSA and 0.1% Pro-Clean 400 |
| Preservative | 0.1% Pro-Clean 400 |
| Stabilizer | 0.2% BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Controls and Markers antibody; Tag Internal Control antibody |
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