ARG57235
anti-5-methylcytosine / 5-mC antibody [RM231]
anti-5-methylcytosine / 5-mC antibody [RM231] for Dot blot,ELISA,ICC/IF,MeDIP and Other
Overview
| Product Description | Rabbit Monoclonal antibody [RM231] recognizes 5-methylcytosine / 5-mC |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, ICC/IF, MeDIP |
| Specificity | This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM231 |
| Isotype | IgG |
| Target Name | 5-methylcytosine / 5-mC |
| Antigen Species | Others |
| Immunogen | BSA-conjugated 5-methylcytosine. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol and 1% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (5) Click the Picture to Zoom In
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ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
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ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ELISA image
ELISA: Titration curve of ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231]. Antigen: The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA.
Secondary antibody: An alkaline phosphatase conjugated anti-rabbit IgG.
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ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] Dot blot image
Dot blot: Double stranded DNA using ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231]. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231].
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ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ELISA image
Direct ELISA: The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. 1 µg/ml or 3 µg/ml of ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] was used as the primary antibody, and a HRR-conjugated anti-rabbit IgG as the secondary antibody.
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ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] MeDIP image
MeDIP: ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 µg of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
