ARG51676
anti-14-3-3 zeta phospho (Ser58) antibody
anti-14-3-3 zeta phospho (Ser58) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes 14-3-3 zeta phospho (Ser58) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 14-3-3 zeta |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL4; 14-3-3-zeta; Protein kinase C inhibitor protein 1; YWHAD; KCIP-1; HEL-S-3; 14-3-3 protein zeta/delta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | YWHAZ |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 28 kDa |
| PTM | The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. |
Images (5) Click the Picture to Zoom In
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ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody WB image
Western blot: 30 µg of Rat brain lysate stained with ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody at 1:500 dilution.
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ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody.
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ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with UV stained with ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody.
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ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody WB image
Western blot: 30 µg of Mouse brain lysate stained with ARG51676 anti-14-3-3 zeta phospho (Ser58) antibody at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-14-3-3 zeta phospho (Ser58) antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-14-3-3 zeta phospho (Ser58) antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
